Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a Wangshuibai derived population
Naji. A. M Moghaddam. M Ghaffari. M. R Irandoost. H. P Farsad. L. K Pirseyedi. S. M Mohammadi. S. A Ghareyazie. B Mardi. M
Journal of Genetics and Genomics ; 2008 [Vol.35] Pages:625-629
Abstract
A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat,have been previously identified in the Sumai 3/Stoa population. In this study, we used a Wangshuibai(resistant)/Seri82 (susceptible)derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwm493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in Wangshuibai at 96 h after inoculation was 2.6 times higher than Seri82. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
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