Use of microsatellite marker Pi26 for rapid discrimination of five Japanese genotypes of Phytophthora infestans
Akino. S Baba. Y Kondo. N
Journal of General Plant Pathology ; 2009 [Vol.75] Pages:349-352
Abstract
Microsatellite markers were tested to rapidly discriminate five Japanese genotypes (US-1, JP-1, JP-2, JP-3, and JP-4) of Phytophthora infestans. Collected from 1958 to 2007, 111 isolates of Japanese P. infestans were examined using a fluorescent-labeled primer and capillary electrophoresis. Microsatellite marker Pi26 generated specific products for each genotype without any differences in terms of isolation area or year for a particular genotype. The Pi26 marker is a powerful tool for obtaining information on the structure of Japanese populations of P. infestans.
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