Full Text                                                                            Full-Text Online        Detection of Phytophthora nicotianae by PCR Grote. D      Bertolini. E      Cambra. M      Olmos. A      Kofoet. A      Tuset. J. J       Eppo Bulletin ;  2008  [Vol.30]  Pages:539-541 Abstract Phytophthora nicotianae is an important soil-borne pathogen. The classical determination based on morphological or physiological characters is time and labour-consuming and is taxonomically unsure. Two specific primers designed from the spacer regions ITS1 and ITS2 were used to detect P. nicotianae by polymerase chain reaction (PCR). A PCR fragment of 737 bp was obtained from all P. nicotianae isolates assayed, but not from other Phytophthora spp. or other genera. Thus, the PCR characterization was species-specific. Polyclonal antisera against mycelium and zoospores were used for comparison and were only genus-specific. Keywords pcr phytophthora nicotianae