A Phytophthora sojae gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induced in host infection and its anti-oxidative function in yeast
Juan. Z Yuanchao. W Gui. S Xiaobo. Z
Chinese Science Bulletin ; 2006 [Vol.51] Pages:1316-1323
Abstract
Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a multifunctional protein well defined in eukaryotes, especially in mammalian and
Saccharomyces cerevisiae. Using the method of suppression subtractive hybridization (SSH), we identified a Phytophthora sojae cDNA coding GAPDH, which was up-regulated during the early stage of soybean infection. The termed PsGapdh gene possessed three copies in the P. sojae genome. Its amino acid sequence harbored overall conserved
domain of GAPDH, homologous closest to GapC1 of Achlya bisexualis (oomycete) and adjoined to GapC2s of Odontella sinensis and Phaeodactylum tricornutum (diatom), on the C- branch of subfamily GapC in phylogeny tree of GAPDH. The transcriptional level of PsGapdh was up-regulated throughout early infection. Heterogenous expression of PsGapdh in the yeast tdh1-deleted mutant could rescue growth arrest under continuous exposure to H2O2. These results indicated active roles of PsGapdh in pathogen-host interaction and anti-oxidation.
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