N605ab, a specific molecular marker for Phytophthora infestans, distinguishes three genotypes in Japan
Akino. S Shirasawa. Y Kondo. N Naito. S
Journal of General Plant Pathology ; 2008 [Vol.74] Pages:125-127
Abstract
Random amplified polymorphic DNA (RAPD)analysis using the OPG-06 primer generated specific patterns for Japanese genotypes US-1, JP-1, and a new A1(JP-2, JP-3, and JP-4) of Phytophthora infestans. N605, a specific RAPD fragment, was cloned and sequenced. PCR primers BD1/BD2 were constructed based on the N605 sequence and were used to clarify the genotypes. PCR products using the BD1/BD2 primers (N605ab marker)easily distinguished the new A1 from US-1 and JP-1. This technique provides a simple and effective method for rapid genotype discrimination that can be used in ecological experiments and forecasts for the occurrence of late blight.
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