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General Info
| Code | Path XVIII | |
|---|---|---|
| Title | Isolation and evaluation of antimicrobial compounds from bacterial endophytes against major pathogens of spices | |
| Crop(s) | General | |
| Start year | 2008 | Closed |
| End year | 2012 | |
| Investigators | Name | Man-months |
| A Kumar | 6 | |
| Santhosh J Eapen | 6 | |
Objectives
- To select strains harboring known antimicrobial gene(s) e.g. DAPG/PCA etc using PCR based (marker assisted) selection
- To isolate cyclic lipopeptides (CLPs) from bacterial endophytes
- To evaluate the activities of cyclic lipopeptides on different growth stages of Phytophthora capsici, Pythium myriotylum, Ralstonia solanacearum Radopholus similis, Meloidogyne incognita in vitro
- To evaluate the metabolites in vivo for disease suppression and growth promotion of host plants
- To purify/identify the potential metabolites using chromatography (HPLC/TLC/GC-MS)
- To manage fungal, bacterial and nematode diseases of spice crops using biosurfactant producing bacterial antagonists
Salient Achievements
- Isolation of primary and secondary metabolites from endophytes
- PCR based screening for production of antimicrobial metabolites
- In vitro and in vivo evaluation of aqueous and organic fraction against fungal, bacterial and nematode pathogens
- Genotypic characterization Pseudomonas aerugenosa
Research Papers
- Aravind, R., Kumar, A., Eapen, S.J. and Ramana, K.V. 2009. Endophytic bacterial flora in root and stem tissues of black pepper (Piper nigrum L.) genotype: isolation, identification and evaluation against Phytophthora capsici. Letters in Applied Microbiology 48: 58–64.
- b. Aravind, R., Eapen, S.J., Kumar, A., Dinu A. and Ramana, K.V. 2010.Screening of endophytic bacteria and evaluation of selected isolates for suppression of burrowing nematode (Radopholus similis Thorne) using three varieties of black pepper (Piper nigrum L.). Crop Protection 29: 318–324.
