LOCUS       EY195367                 350 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21e07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491282.1| Calponin homology (CH) domain
            containing protein family member [Caenorhabditis elegans]
            pir|T29467 hypothetical protein F28H1.2 - Caenorhabditis elegans
            gb|AAB52338.1| Calponin protein 3 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY195367
VERSION     EY195367.1  GI:159498513
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 814 was extended to value 815 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 542
            PolyA tail found inside read...SR being truncated to 501
            Potential full insert read...vec-to-vec length: 422
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtcg cgaagagcca gggagtgccc accgaggaga ccttccagag cgtggacttg
       61 ttcgaggcac gtgacctcta ctccgtgtgc atgaccctgt tgtcgttggg ccgaattatg
      121 gagaagaagg gaaagccgaa cccattctct ggatgaagag tagaagtgag tgcagcaaaa
      181 gacggacaga gcatgtgcta tcgctcccat tcgagaactc cccgttttgc gaattttctc
      241 cccgtgtgcg accttgcaac aataccgagg cgttaactgt tttcccctct ctctcctctc
      301 aaactgtggg catttgaaaa tagcgatgcc ggaataaatg gccaattcca
//