LOCUS EY195366 525 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195366 VERSION EY195366.1 GI:159498512 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 525) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 825 was extended to value 825 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 694 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..525 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ggcacgtaat tcaactggtc tttttgctgg gcggcaattg gcaactttat 61 ccgacagaag catgataatt ggtggagccg ccctttgccg acgcactttt gcagccccac 121 ccccccatcg ggacacggta ttcgaggtag aatcggacga ggacttcgag aatggggtat 181 accatgccga aaaaccagtg ctcatgcagt tttacgccga ttggtgtgga ccctgtcaga 241 atttggcacc gaggttgatt gccaaagtga atggacagga tgggaaggta ttgctagcga 301 gagtgaatgt agagggttcc gccggatttc tcgcggaaca gtttgatgta agctcgattc 361 ccactgtgat gtgctggctg cgaggagagg tggttgaccg ttttgagggc gacgtggaag 421 acacgaagat tgaccaaatc atatccaaat tggtggaata tcaaactgga aatgaatgat 481 aaagggcttg aacagccatt tagtagacga aaaaaaaaaa aaaaa //