LOCUS       EY195364                 474 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_500582.1| GTP-binding protein like (21.7 kD)
            [Caenorhabditis elegans] sp|Q23445|SAR1_CAEEL GTP-binding protein
            SAR1 pir|T29706 GTP-binding protein ZK180.4 [similarity] -
            Caenorhabditis elegans gb|AAB52968.1| Hypothetical protein ZK180.4
            [C, mRNA sequence.
ACCESSION   EY195364
VERSION     EY195364.1  GI:159498510
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 853 was extended to value 854 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 665
            PolyA tail found inside read...SR being truncated to 624
            Potential full insert read...vec-to-vec length: 546
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga cgccattgta tttttggtcg atgtagccga cctggaacgt attcaggaag
       61 caagggagga attgtggagt ctgatgcagg atgaacaggt ggcaagtgca cctgtgcttg
      121 ttttgggcaa taagatcgac aagccgaatg ctctcagcga agaccagctc aagtactacc
      181 tcggcatcca acaatactgc acaggaaaag gccaagttgc gcgctcagat ctggccactc
      241 gtcctttgga tgtgttcatg tgctcagtcc ttaggcgaca gggttacggc gaaggattct
      301 gttggctctc acaatatctg gactgattga acgcgcctcg gaagttgaaa attgacacaa
      361 aaagtaagga cgactccaat cgcaacaaat catttcatat tatttctgta ctacacctat
      421 tttcgattca tcttatctct taaacaatgt caatgttaaa aatcatcggt tgca
//