LOCUS EY195363 239 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAP59456.1| cathepsin B precursor [Araneus ventricosus], mRNA sequence. ACCESSION EY195363 VERSION EY195363.1 GI:159498509 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 239) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 432 PolyA tail found inside read...SR being truncated to 390 Potential full insert read...vec-to-vec length: 311 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..239 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggct cgggggccat gccgttcgca ttattggatg gggcgaggct agcggtcaga 61 aatactggct ggtggctaat tcgtggaaca ccgattgggg cgagaagggc ctattccgca 121 tacgtcgcgg ctccgatgaa gagcgcatcg aaacattgca aattgcattt gggacaccaa 181 agatttaaaa tcggcgaatt gacttgtaaa agatggatag taaaatattt cttttgcca //