LOCUS EY195362 581 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195362 VERSION EY195362.1 GI:159498508 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 581) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 788 was extended to value 789 (,) putative full insert read: vector-to-vector length is 608 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 755 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..581 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac attaagtgca atcaattcgc cacataatgt gatgtcaaaa tttaaatctg 61 aaacttggat ctattgtaac aacatcggat ggaccatcag ctggtgggag caagcaacaa 121 gagatggaag acgatacacc ggccaaagag caagaggaag aacgcgaact gggcgaagag 181 gatggatttg cgcaaaacca tcacaatagt caattttcgt agcctcccaa cgccaacagc 241 cgcctttctg gcacattatg tgaagagtga tcgtccattc catgcgctgt tccgtcgtcg 301 acctgttcct gcgcacttgg gggagaccaa ggccaaatcg atgtaattta attgaacaaa 361 aaattaatgc agttcacggc ttgtctttca tgccttggat gaactcttca ttcattcgga 421 atcaaccatg gccacgttac gtcaactgga agatttgccg gagaatgtgc tggctagacg 481 gaggctccag acagttcgag ccaacgaact cgtcaattag agaaaccaca aaatataaag 541 ttgacatttt atgaataaat atatgaaaaa aaaaaaaaaa a //