LOCUS EY195347 623 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_777285.1| RuvB-like protein 2 [Danio rerio] gb|AAL18005.1| RuvB-like DNA helicase reptin [Danio rerio], mRNA sequence. ACCESSION EY195347 VERSION EY195347.1 GI:159498493 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 623) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 831 was extended to value 832 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 835 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..623 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag aaaggcagca ggtctcattg tccggatgat taaggatggt ctaattgccg 61 gaagagctct actgttggtt ggggagccag gcaccgggaa aactgcagtg gcaatgggga 121 tttctaagtc aattggcaaa gataccccct ttgtgtccat agccgctagt gaagtctttt 181 cggttgatat cggcaagact gaggcgctaa cccaagcatt tcgaaaagca ataggagtac 241 gaattaaaga agaaacagag gtacttagag gcgaagttgt tcaagtagag attgaccgtc 301 ctacaaccgg cgtcggaccg aaagttggaa aattaacatt aaagaccaca gacatggagg 361 caatctacga tatcggcaac aaaatgacgg aatcgttgat caaggagcga attacggcag 421 gcgatatcat tcaaattgac aaggcaactg ggacaatctc aaaaattgga cgatctatcg 481 ccaaatcgaa ggattacgat gcacttggtc catcaacaaa tctagttgct tgcccatctg 541 gcgaaataca atctcgcgta gaatccgtta cacgatatcg ttacacgata tcgatgccat 601 aaattctcgt atgcacggat att //