LOCUS EY195346 370 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21c05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195346 VERSION EY195346.1 GI:159498492 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 370) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 563 PolyA tail found inside read...SR being truncated to 521 Potential full insert read...vec-to-vec length: 442 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..370 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcac gaggatagca ctgcgttcat ttcggacgag tctcgcagag tggcgctgcg 61 atggaagaac cgagtgatcg cagcactgtt catgttctac aagtcaaatg gacacaaatc 121 ctaccccgag cgcttcggca acgttgtcct actcatttcg ggaatcgtgt cggcgggcaa 181 tcccatttac cattaattca tttccaaccg aacagctaat tttccagcct ctgccacctt 241 ggagtcctat cagatcatgc ggcttttcaa gtttgccagg ttcgaccgga tatcggagca 301 gcttctcttt gacgtagacg caaactgact tcaacatata cttacatact tataaagaga 361 gtaatattca //