LOCUS EY195342 258 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21b12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195342 VERSION EY195342.1 GI:159498488 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 258) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 451 PolyA tail found inside read...SR being truncated to 409 Potential full insert read...vec-to-vec length: 330 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..258 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaga agaacattgt gcgcgccgcg gagagcatca aggacaagct gggcaacaag 61 ggctacgcca agttggagga ggagttcctg tgagcggaga caggaatgcg gaatgacaga 121 gcacacgcct agtcaacaaa caaacgaaac atgggacaaa caccgatgca acagatcact 181 tgtttaatcc acatttatcg atcgaatgtc catatcacgg aatcacatgg ataagctcaa 241 gcattcgggc aactagga //