LOCUS       EY195340                 541 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_498520.1| Elongation factor EFT-3 (50.7 kD)
            (eft-3) [Caenorhabditis elegans] ref|NP_509323.1| Elongation factor
            Tu (50.7 kD) [Caenorhabditis elegans] sp|P53013|EF1A_CAEEL
            ELONGATION FACTOR 1-ALPHA (EF-1-ALPHA) pir|T16218 translation
            elonga, mRNA sequence.
ACCESSION   EY195340
VERSION     EY195340.1  GI:159498486
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 808
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtt cgaggccggt atctccaaga acggccagac tcgcgaacac gccttgctcg
       61 cccaaactct gggagtgaag cagctcattg ttgcgtgcaa caagatggac accactgagc
      121 cgcccttctc cgaggctcgc tacaccgaag tcatgaccga ggtgtccagc ttcatcaaga
      181 agatcggata caacccggcc gcggttccgt tcgtgccgat ctccggcttc aacggcgaca
      241 acatgttgga ggcctccgac cgcatgccct ggttcaaggg atggaacgtc gagcgcaagg
      301 aaggcaatgc ctccggaaag actctgctgg aggccttgga cgccattctg cctccgcagc
      361 ggccaaccga caagccgctc cggctgcctc ttcaagacgt gtacaagatc ggaggtattg
      421 gcacagtgcc cgtcggccgt gtcgagaccg gagtgctgaa gccaggaatg gtcgtgacct
      481 tcgccccgca tggcgtctcc accgaggtga agtcggtcga aatgcaccac gagtcgctgc
      541 c
//