LOCUS EY195339 520 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to pir|T15810 hypothetical protein C46F4.2 - Caenorhabditis elegans, mRNA sequence. ACCESSION EY195339 VERSION EY195339.1 GI:159498485 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 520) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 814 was extended to value 814 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 818 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..520 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag cactggctcc gttggccccc cgctccgttg ttgcgagatc aagctccgcg 61 agtggactga agctggctac accccatcca actcgccgcc gcaaggagaa attctgatcc 121 atggggacaa tgtggccctg ggctactaca agaacgagga gaagaccaag gaggacttca 181 tcgagttgga cggaaagcgc tggttcgcca ccggggacat tggggagttc cgaagcgatg 241 gctctctgtg catcattgac cgcaagaagg acctcatcaa gttggctcac ggcgaataca 301 tttctctggg caaggtggag acttcgcagc tgaccaaccc gaacatcgac aacatctgcg 361 tctacggaaa cccccaccgc gactacttga tcgcactggt cgtgccgaac aggaagaatc 421 tcgaggccct cgcggagaag aacgacatca gtgagcccag ctgggacaag ctttgtgcga 481 acaaagaact cgcaaaggtc ctgcagaagg agctccaaga //