LOCUS EY195337 603 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAD38835.1| histone h2A.2 [Oikopleura dioica], mRNA sequence. ACCESSION EY195337 VERSION EY195337.1 GI:159498483 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 603) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 624 (,) original QR value of 828 was extended to value 829 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 772 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..603 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga aagggtggga aagcgaagac tgggggaaag gctaagtcgc gatcttcgcg 61 tgccggactc cagttcccgg ttgggcgtct gcaccgtcta ctgcgcaaag gtcattacgc 121 ggagcgtgtg ggcgccggcg cgccagttta tctggctgcg gtgctcgaat acttggcggc 181 cgaagtgctc gaactggccg gcaacgctgc ccgcgacaac aaaaagtcac gcatcaatcc 241 ccgccatctc cagctggcgg ttcgcaacga cgaagagctg aacaagctgc tctccggtgt 301 caccattgcg cagggcggtg tgttgccgaa catccaggca gtgctgctgc cgaagaagac 361 acacgtcgag ggtggccagt gagctcggtt ggaggaacga gagaagtgac gcgaaaacca 421 aaagaagttg tgacgtattg agctcggcga cgtttatttt tatgtaacta ttttcgagat 481 tcattgacga acataccatg tatttttaag tctcgcaaac gtgcatcaaa catgtcttca 541 tattcatata tcattaacac acctcctgtg gaaaaagaaa tcatatagtc aaaaaaaaaa 601 aaa //