LOCUS EY195335 534 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to dbj|BAA21484.1| cdc2-related kinase [Bombyx mori], mRNA sequence. ACCESSION EY195335 VERSION EY195335.1 GI:159498481 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 534) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 835 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..534 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga gcgtgtcgtt cggtgagcga gttcgaaaaa attcgccaac tcggcgaggg 61 tacttacggc gttgtgtacg cgggcaagga caagcgcaac ggacaaacgg tagcactgaa 121 gcagatccgg cttaatctga agaaagaggg ggtttccgtg tcggtgatgc gcgaaatcca 181 gtccctgatg gacattgagc acgagaacat tgtgcgcctg ttcgacgtgg tggttggccg 241 acgcattgat cgcatcttcc tcgtcatgga gtattgtgat caggatctcg cctcactgct 301 ggacaatatg acttcgccat tcactgaggc gcaagtgaag tgtctgctgc tgcagctctt 361 ccagggaatg caccatttgc acagtcgcta tattgtgcat cgagacatca aggtttccaa 421 tttgttgctg accgagtgcg gtgtgctcaa aatcgccgac ttcggcctgg ccagactgtg 481 cggcgatcca gccacccaaa tgacaccgcg cgtggtcaca ctttggtatc gcgc //