LOCUS       EY195331                 564 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195331
VERSION     EY195331.1  GI:159498477
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 783 was extended to value 784 (,)
            putative full insert read: vector-to-vector length is 605 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 756
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac agaacgcaac aggtttggag cgaaatcaaa tgaacttcct atcttccgac
       61 ctatacacat tcagagtata atcgacgtgg ctaatttgga gaacagttcg cttgactttg
      121 acgctattca acggtctcgc ctctacgctg aggctataaa aagacacgac gcccagacgg
      181 acagacgcaa gggtatgctt accggcagtg aaagagccgc cgattctgaa atttcccatt
      241 ttactctcct ctcaaatgtt ggaactcgtg cgcatgaact gattggacaa ccggcgcaac
      301 gtttggatca agaagaactg acattgctct gtcgtgccag tgagcttttc ttggaggcgc
      361 atcggttggg cgcccaaatc gagtgcaaag aggatctgat tgtgtacatg tcactggacg
      421 attgacatag agtgaatgaa tgacaaatga attaaggcaa tcctgttttg ctcattcatc
      481 acaggtctct ctttttcttc gctgtactcc tcccatttcc atttgccttc gattctctga
      541 accaaaaata tgcgtaaaaa aaaa
//