LOCUS EY195328 497 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_494846.1| Serine carboxypeptidase precursor family member (53.6 kD) [Caenorhabditis elegans] sp|P52717|YUW5_CAEEL Putative serine carboxypeptidase F41C3.5 precursor pir|T16316 probable serine carboxypeptidase (EC 3.4.16.-) F41C3.5 precursor, mRNA sequence. ACCESSION EY195328 VERSION EY195328.1 GI:159498474 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 497) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 835 was extended to value 835 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 690 PolyA tail found inside read...SR being truncated to 647 Potential full insert read...vec-to-vec length: 569 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..497 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcag tgccgatgtg cgcaaggcgc tgcacattcc cgccaatgtg ggccactggg 61 acatttgcag cgatgaaatc accgagaact accagaagca atacgacgac acgtcgccat 121 tcttcaagac cattctgggc gctcatgtgc gcactctgct ctactacggg gacacggaca 181 tggcgtgcaa cttcatgctg gggcagcagt tttccgccag actgggctac aaacgcgcca 241 tcggaaaaca gccgtggaaa ttcgaccgcc aagtcggcgg attcaaaacc atctacgcga 301 agggattgac ctatttgacg gtacgcggag cggggcacat ggcgccgcag tggaaggcac 361 cgcaaatgga ctacgtgatc agccagtttc tgctgaacca ccctatttga ggagtgagga 421 ctgaggagga aggaaatagt gtcaaccccc agttatgcta tctacttgcc gacgagcgca 481 taaattaagc atctgca //