LOCUS       EY195327                 375 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21a07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195327
VERSION     EY195327.1  GI:159498473
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 569
            PolyA tail found inside read...SR being truncated to 525
            Potential full insert read...vec-to-vec length: 447
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gaaggactcc cccaaggcgc ccattccact cgacaaattc aagctgctcg
       61 tgcccgtcct cgaaacgaca ttcccgatga caaagggatt cggcgcggac ctggacgcgg
      121 cagacaccaa tggcgatgga ctgttgagca tggacgagta catcgcggaa tgggtcaaat
      181 tgagcgttcg cagcgtggac aagaatgggg acggagttgt gtcgaaggcg gagaacgcag
      241 aggccattgc gggcaaggga ttcggattcg gtcgcaaacg gaagtgactt gggcgaaagg
      301 gaagcaaaga aaaagtgccg tttgattgac atttatgtaa tcttcacttc acttcacttc
      361 acgattattt actga
//