LOCUS EY195326 551 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21a06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAA74624.1| elongation factor-1d [Sphaerechinus granularis] emb|CAA74625.1| elongation factor-1d [Sphaerechinus granularis], mRNA sequence. ACCESSION EY195326 VERSION EY195326.1 GI:159498472 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 551) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 824 was extended to value 825 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 830 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..551 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcat ccatggtttt cttccatcgc aagaagatca atacgtgttc aagtgcttca 61 cagctgcacc tgcccaaaaa tacgcgcaca tcacgcgttg gtacaagcac atccagagct 121 atgaagacgc agagcgctca acttggccac aaaaagccgg tggacctcct tcagccagtg 181 ctgccaaaca atcttctccg cccaagggag cagccgctgc cgccggcgga gatgacgatt 241 tcgacctgtt cggcgatgaa gacagcgatg acgaagagaa gaagcgaatc accgaggagc 301 gcttgaaggc ctacgcggag aagaagtcca agaagcccgg ccccatcgcc aagtccaacg 361 tgatctacga tgtcaagccc tgggacgaca ctgtggacat caaggagatc gagaagaatg 421 tgcgctctat tcacacagag gggcttgtct ggcgcacttc caagatcctg ccagtggcat 481 tcggcgttaa gaagcttcag atttgctgct gtgtggagga cgaaaaggtc tcaactgact 541 ggctagagga g //