LOCUS EY195324 234 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21a04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAL09384.1| glutathione peroxidase [Haemonchus contortus], mRNA sequence. ACCESSION EY195324 VERSION EY195324.1 GI:159498470 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 234) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 747 was extended to value 747 (,) putative full insert read: vector-to-vector length is 370 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 519 PolyA tail found inside read...SR being truncated to 385 Potential full insert read...vec-to-vec length: 376 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..234 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt gacgccatca agtggaactt cacaaagttc ctcgtgaacc gaaaggggaa 61 tccagtggct cgttttgcgc caaccaccga gccgaacaaa ctgcacgcgg acattgagaa 121 gttgctcgag aaacaggcat aagcgaagag aagagaacca gttagccaga caaaatgatg 181 actacataaa ataaatactt aatttattcg ctatgatgac acactctatt ttca //