LOCUS EY195323 292 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_310539.1| ENSANGP00000017317 [Anopheles gambiae] gb|EAA06275.1| ENSANGP00000017317 [Anopheles gambiae str. PEST], mRNA sequence. ACCESSION EY195323 VERSION EY195323.1 GI:159498469 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 292) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 486 PolyA tail found inside read...SR being truncated to 443 Potential full insert read...vec-to-vec length: 364 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..292 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt catcgtgctg atcggctcgc tgggagaaca ggcggccctg ttgcagcggg 61 ccctacccgc ggggaaggca tttgtcgcca tggacgcggc acagatcccc gagatcatgc 121 agcagatatt cgcttccaca atcatcagat agggagacta ggaatcactc catccatcat 181 ctgctgttca gtcccccaca gagatagaac aagtgcctcg tgttgctcaa aagttttgtt 241 gtaccgttgt gttctttgca cttaaacagt gtttaaattt gatttccatt ga //