LOCUS EY195318 651 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAB59955.1| acid phosphatase-1 [Drosophila subobscura], mRNA sequence. ACCESSION EY195318 VERSION EY195318.1 GI:159498464 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 651) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 811 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..651 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ggcaaagtac gcggagttat ttgaattctt gcgcactgtg ttccgttcaa 61 aagagccaat cacattgcac aaggcggcca agttgagcaa tatcagacgg gagattgcgc 121 ataacctgac acaaccggaa tggggggtgt atcggcagtg gccccaatac gggaatacaa 181 cgacactcga gttgatcacc gagttgaagc gtttggaacg cacgcgtgag tttgcgcatc 241 cagaactggc cttcttgaag ggcggcttcc tccttggcga ctggatccgt cgtgtcgaga 301 cgcatttgcg cctagaatca ccgcaagagg caaaaaaggc agtgctttat tcggcacatg 361 acggcactct acaagccctc ctttatgcaa tgggattggg cgacgaccag ttgattccgt 421 acgcagcgtg cataatgatg gagatttgga gtgatcaacg acgaaatgga acccttgaag 481 ttgaaattta ttaccgcaga gacagtcaat tgcgtcgcct aaatttgaca gagtgtgggt 541 taaactcctg tgaggcaaag atggctacca ctttgttaag aaagaaggca atattcgaca 601 gacaacaact gtttaagata tgtgaaaaga atggatattg tgcaattaca g //