LOCUS EY195317 385 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195317 VERSION EY195317.1 GI:159498463 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 385) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 801 was extended to value 802 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 581 PolyA tail found inside read...SR being truncated to 536 Potential full insert read...vec-to-vec length: 457 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..385 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggata taaatttata ttaatatagg tgaaatttta attataatta tgttaataat 61 tttataaaat ttaaaatttt aatttttaac ttaggttaga cactaattaa tgaaatttaa 121 taattttctt tagtaaattt ttgtaaaaaa agaaaaaatt aaggcagaaa tattaagaat 181 ttttggagtt tgaagattaa ttagagaaac ctctttatta tctttattta ttgtttcttg 241 tatgtttatc gtaaaattaa ttattttatt ttttattaat atttttatat aaaattagta 301 gataaatatc gggattataa tggagttttt ttttaactac aatattataa atttttattt 361 gaatattata gtaattatat attga //