LOCUS EY195316 324 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195316 VERSION EY195316.1 GI:159498462 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 324) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 518 PolyA tail found inside read...SR being truncated to 475 Potential full insert read...vec-to-vec length: 396 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..324 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ttttgtaatt atgattttag gttataaata attaatgaag tataaactat 61 taatgttata ttttttagat aaattaattt ttctagtaat ttatttttag ataaattaat 121 gttccagaaa tatcggctag acattattat ttttaactaa aaacttttta aattttattt 181 taatttatat aaatttatat taatataggt gaaattttaa ttataattat gttaataatt 241 ttataaaatt taaaatttta atttttaact taggttagac actaattaat gaaatttaat 301 aattttcttt agtaaatttt tgga //