LOCUS EY195315 388 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195315 VERSION EY195315.1 GI:159498461 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 388) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 582 PolyA tail found inside read...SR being truncated to 539 Potential full insert read...vec-to-vec length: 460 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..388 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat tccattcccg aaggtattct cgatattcta cacacatttt tttcaactca 61 ccaaggagaa ccaaagcaga aatggcctgc gagtgcagct gctgcaagaa caatgtgtgc 121 tgcaagagcg cctgctgttc tggcgacgac tgctcctcat gcagctgccc gaactgcgag 181 tgccccaagc agaagggaca gtgctgcccg cagcaacagc agggaaaggg atgctgcgca 241 cagaagtaga tgggaagaag agagcagaaa aaacggaagt cgatgcctgc agatgatgat 301 gacctaacca aaatggcaaa gataccataa cacacttttt ttgacaaatt tttgggaaaa 361 atcgaaaagc taaaataatt tttatgca //