LOCUS EY195313 657 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195313 VERSION EY195313.1 GI:159498459 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 657) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 825 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..657 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca gcgacgtagc gaccacagaa ggcagatgac accaactcta aaaaaataag 61 catggtggaa ttcgacgact ctcgagacat gaatagaaaa acgtccaatt aataaatgtc 121 gtcaaacaat ccaaatctcg caggaaagcg gaaggttgcg ctaaatgagt gcaaaaagat 181 atacatccaa cgagattaca gccaagcgtt gattgtcaga ttctgcacag aatttccgga 241 ggaacttgaa caatacattg aacgggaagt gtggctgaac ttcatcaatg atctgaacca 301 agtttatctc gaagccgaga cgttgtcagc gcgtgcttat gcagagaact tccttggcct 361 tctcacatgc catatttcac ggttatgctt aaaatcccaa tggcatcgaa agctagaaga 421 ggccgaattg ctgatcgacg atgccaacca gcagagcctc ataaagaacg ggatctacgt 481 cacaaaccct ctggagaagg ggttcagagt ggttgaagtt gttctcctcg aggagccctt 541 gcctctagtc acggtggttg gcgacaaaac cgaaaagagt tccgcaccca cgatctcgag 601 gagcccttgc ctctagtcac ggtggttggc gacaaaaccg aaaagagttc cgcaccc //