LOCUS EY195311 478 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAC32452.1| FMRFamide-related peptide 2 [Globodera pallida], mRNA sequence. ACCESSION EY195311 VERSION EY195311.1 GI:159498457 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 478) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 796 was extended to value 797 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 670 PolyA tail found inside read...SR being truncated to 630 Potential full insert read...vec-to-vec length: 596 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..478 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga ctcagccagt ctgtcattca ttctcaaaag aaagcaatcc ccagtcattc 61 ctaccaattc ccaaatttca ttaaaatcct tcatccttcg aaaaaatgtt ccgcgttcct 121 acaattgcca tcctggccgc gatgtccgtt tgcttcgttg tgttaagcgc agaagtcggt 181 gcgcaaaagc caaacctgat tgccgaaaga actcttgaga acgggaggag tgcgaatggt 241 ctcgatggcg aggtcatcga caaagtcgaa tcgcaactcc ttggagcgct aagtctactg 301 caagcttacc aagaagtacc cccggttccg aaattcgcgg agaagagacg aaacaagttt 361 gagttcatcc gcttcggcaa aagacggtga gcgagttgtg cgagcagaat gatgggggaa 421 agaatcagtg cgacgagaac taccatttga aatcgacaat aaagttcttt tcgtgtta //