LOCUS EY195310 314 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_506352.1| Transthyretin-like family member precursor family member (17.8 kD) [Caenorhabditis elegans] pir|T20267 hypothetical protein C56A3.2 - Caenorhabditis elegans emb|CAB01136.1| Hypothetical protein C56A3.2 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195310 VERSION EY195310.1 GI:159498456 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 314) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 789 was extended to value 790 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 509 PolyA tail found inside read...SR being truncated to 465 Potential full insert read...vec-to-vec length: 386 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..314 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gttcaagatt cccaactcgt acgtcagtga gggagccacc cccaaaaaaa 61 catttgacat cggcacgctg aatctggaga ccatcttcgc caacgaggag agggagttga 121 tcgtgagcaa gaaaaggcga cacttgcacc actaccgggg cggcgagaaa ttcaactttt 181 tcgttcgtct ctaggtggca aagaaagaag aatttggata atggataagc caaaacaaac 241 aaaacaatac aaatcgaccc tctttcgact tgtcaaattg ctttttaatt gatttgcaat 301 aaagtttttg caca //