LOCUS EY195309 167 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195309 VERSION EY195309.1 GI:159498455 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 167) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 819 was extended to value 820 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 386 PolyA tail found inside read...SR being truncated to 318 Potential full insert read...vec-to-vec length: 239 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..167 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga aaatggagag aactgagggc attaacataa ctaagcggac acgaagcact 61 gcgcaaatac gagtgaccga ataacctctg acatcatcgt tcttcataaa tcataacaat 121 attccagaaa tttcaagaga tttataaata aaaattaaat tactgca //