LOCUS EY195307 560 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195307 VERSION EY195307.1 GI:159498453 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 560) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 817 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..560 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac tggtcacagt gccagcgctg gagcatgaag ctaaacccca ccaaagaacg 61 ttctatttga acccgcaggg aaaaacatcg gtcagtattc tccatggtgt gtgaaagtct 121 ggatgccagt gctataactt tattgcagag tttgtacaga aagtactcaa gtgcacggtt 181 cgctacttgt ataacgaaac aaggtttcgt agcccggatg tgcaatttat attgttttgg 241 ctattcgccc cataggagta gcgccactcc gtaccattgt gttgccaaac tggtaaacaa 301 cccttgcgcg ccaattatgg acgacccaaa tggggacaat gcaaaaaaag aaaagagtct 361 cgcagcggac tgcattcgca aaaagttggc aatcattcaa gccatgcacc agaaggaagg 421 tggcacgcag ctgcaggcag aaataaaaga agaactggat gctgagaggg acagtgaatt 481 cattgaactc ggccaaggct acggagcgag aaaaaagagc gcaaagcttt tagcggccaa 541 agatgccctt catcgtctca //