LOCUS EY195306 265 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_510149.1| Putative protein family member, nematode specific [Caenorhabditis elegans] pir|T18962 hypothetical protein C34E7.4 - Caenorhabditis elegans emb|CAA91790.1| Hypothetical protein C34E7.4 [Caenorhabditis elegans] emb|CAA91795.1| Hyp, mRNA sequence. ACCESSION EY195306 VERSION EY195306.1 GI:159498452 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 265) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 850 PolyA tail found inside read...SR being truncated to 416 Potential full insert read...vec-to-vec length: 337 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..265 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc catcaaggag ctgaacgacg ctctgcacgg cgccaagaag ggcatgatcg 61 ggtgccattg gatctacgcg accgtgagaa gcgtgaacca gtagagagac gcaaggaaag 121 gacacaacat tcattgttgg cacccaacaa caagcgggaa cacggaacaa tcaatccaca 181 cagctcttgc ccctttcacg cattcttctg tctccccgtg aacgtgcgcc ccaaatgatt 241 attcgaagta aagaccattt tttga //