LOCUS EY195305 372 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195305 VERSION EY195305.1 GI:159498451 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 372) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 564 PolyA tail found inside read...SR being truncated to 523 Potential full insert read...vec-to-vec length: 444 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..372 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggct ctgtctcgag gacgagtttg atgagaacca ggcggaccgt gatgtcgaga 61 ccgacccgcc cccctgggtg agcgagtgtc cggcgcaaca gcggcagacg gtcgagatgc 121 tcagcattgt ccgtgtgcgg ctggttccgg tgaagaactc ggtcaaatac gacgtcaaat 181 tggtatcaac cccagactcc agacagctca cctgggtgga catcagcaga gacacataga 241 tgaatgatgg agatattagg catttgcaca cattttgttg actcgttaac ggttaattgc 301 caatctcctg acattgcttt gatgtgattg tggtttacgt ttttctatga gaacaataaa 361 catcgaatgt ca //