LOCUS EY195304 601 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195304 VERSION EY195304.1 GI:159498450 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 601) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 832 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..601 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa aaaatcgcag aaaaagcgag gaagagggcg gaaaaatcaa caaaagccga 61 ggaagcttgt ggatagcgaa cagggctgtt caagcgaggg atgtgaaggt acgggaaatg 121 ggccagaggc agcagcacga caacccacga gtgcagtcgg caaaaagagg aaggccgatg 181 caacggagga cggcctggat ccggccgatc tgaagaagtc accattggaa atgagcgcga 241 caaacagccc attccaactc caagcggagc aacacgagac gtcgttcagc agacgggcaa 301 ttcccaaatg gaagttatcg cgagggcgtc acagggaatg gattctttca ccactggatt 361 ctcgacgctc tgttttctcc gcgactctgc cttccgcaat gagggccgtc tagacacaag 421 tctggccggc cgaattgccc ttccgggaaa gcagtttcca ctattgttgc gcaaatccca 481 gttcgtgagt caaaagtccg accatgtgct gaactggatt cgggtggagg gtcggagacg 541 agcgtttgac gacgcaaaat ctagcggtat ggaggatccg ggggaccaag agcaacagga 601 c //