LOCUS       EY195301                 422 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab19g02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195301
VERSION     EY195301.1  GI:159498447
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 797 was extended to value 798 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 615
            PolyA tail found inside read...SR being truncated to 574
            Potential full insert read...vec-to-vec length: 494
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac acacatttgt ttgtgcgccg ccaactctcc cgagaggagc caacccgcat
       61 cccactgata ctatttctgt atttctgctc actcgctcaa cgccaaacag cgacacaatg
      121 ctgggtctgc agttcgccta cttggagcct gccgccgtcg gcgcccaatc ggaggagcag
      181 ctgctgcaag agtgcgccga agtgaaggac gggttcgggc gtgcggagcg gcagcagcag
      241 ccgacggcag aagcccaagc gccgcagcag cagcagaagc acttcatcgc acgagtgctc
      301 cagcacattg gcagtggcgg ccgaacagcc gaaaatggcg cagacagcaa cagcacacac
      361 aattactgct ctttgccaaa cgatgatgat ccttgaaacg ttgaaacagt aaaaacttct
      421 ga
//