LOCUS EY195299 478 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492601.1| Cytochrome c oxidase subunit Vb (14.7 kD) [Caenorhabditis elegans] pir|T21416 hypothetical protein F26E4.9 - Caenorhabditis elegans emb|CAB03002.1| Hypothetical protein F26E4.9 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195299 VERSION EY195299.1 GI:159498445 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 478) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 669 PolyA tail found inside read...SR being truncated to 629 Potential full insert read...vec-to-vec length: 550 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..478 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg tgttactgac aaatttggcg aaggcacgct ttcaattgac cacacttggt 61 acgaacaagc gttttttcgc ttcggaggct gatcccgatg actacggtta ccaccccgat 121 ccgattgaac acgcttgggg acgggaaagg aaaatgatca ttgcacgtct gatgggcgac 181 gatcgttacg aacccaaagt gtattatcgc gcagaggaat ccacacgaga catgccaaat 241 cttgtgccct gtcactttga cgacaaggtg gtcggctgct tgtgcgagcc agacactgga 301 tacgtgaact actgggttat ccggaagggc aacataaaac ggtgtgaatg cgggcattgg 361 ttcaaaggga tcgaagccga tccagaaagt gtctaaggac atgcacaact tgataggaca 421 cgtcggttta ttgagtttgt aaatcttaaa gttttcattg ataaagtgtt tttagcga //