LOCUS       EY195298                 321 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab19f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499365.1| Putative nuclear protein, with a coiled
            coil-4 domain, of eukaryotic origin (12.2 kD) [Caenorhabditis
            elegans] pir|T23204 hypothetical protein K01G5.8b - Caenorhabditis
            elegans emb|CAB07863.1| Hypothetical protein K01G5.8b [Caenor, mRNA
            sequence.
ACCESSION   EY195298
VERSION     EY195298.1  GI:159498444
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 516
            PolyA tail found inside read...SR being truncated to 471
            Potential full insert read...vec-to-vec length: 393
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtaa agaccaaggc ggaaattgcc ttcataaaaa ggcaagagga gactgcgttt
       61 gagcgtttgt ccaaacgggc gcagaccagc cacagggaga aggtggaacg cttcaacaaa
      121 cagatggagg agctgaccga gttcaacgac atcccaaagg tttcttggac gaagtaattt
      181 cgcttgtggt ctcaggctat ggcgaaggcg gaagaagcga aaaatgctct attgttcatt
      241 ttattcaatt ttattcaaaa tgtgagcaaa caagcgatgg taatgacctg atgactggat
      301 ttggataaag cattctgtat a
//