LOCUS EY195296 625 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_497810.1| DNA helicase [Caenorhabditis elegans] pir|T20430 hypothetical protein E03A3.2 - Caenorhabditis elegans emb|CAA86232.1| Hypothetical protein E03A3.2 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195296 VERSION EY195296.1 GI:159498442 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 625) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 842 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..625 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag gggcttcaaa acgaaacaat gcacaaccag gagaaaaact tgccaattta 61 tgatgaagaa tcatccgggg agcctgtaac ctccacaaat ggctctaatg gtgctgtctt 121 caaattgcct agaaagcgtc cgagtgcaga atttgtaata caacaaggag atgagaaacg 181 caaattggat ggcggtaatg tcacttggag tgactgcgac agcgatgctt cactttcatt 241 caacgcccag caacaaacaa ggggatcgcc tgaacaatcg gatcaaaaac cttccagttc 301 tcaaattgaa caactgaatg aaattattac cattgaagac tatgaaaaat cggtcacaga 361 gctgcttgcc gaattgccgg tgcccggttg tccccaatcg acaatggagc gcagagatga 421 gctgctcaaa gtggtgttca agcacgagaa attcaagaga attgtgcagc ggagcgccat 481 caatgcgatc ctttcgaaat cttcggacgt ctacgtcact ttcccgacgg gcgctggcaa 541 atccctctgc taccagctgc ccgcctgctt ccatcccggc ttgacaattg tgttctctcc 601 gttgctggct ctgatttctg accag //