LOCUS       EY195134                 546 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab17g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195134
VERSION     EY195134.1  GI:159498262
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 634 (,)
            original QR value of 818 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 782
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg tggacgagca cttggaaaag gcggccatcc agatacgtag agatgtgcag
       61 cctaacgaga ggtcttcaat caaccattcg aagagaacgc ggtacagcta cttcgccgac
      121 caaatttacg tttacgctaa cggggagacg ttcaaaaacg tacttgctga cgttaggcaa
      181 tccgcacttt tgaataggat tagtgaactt gatccagcac accgcgacca acttctcgca
      241 ttggcaaagt cgtccagaaa ttaattgact agttattgta attgtaaccc cattcttatt
      301 taccaattag ttattgtaat tgtgacccca ttcttattta ccaagtgcta tgttatttta
      361 gattttaaca ctcagatcac agctaattct catcagagtt ctataacaca ttcctaactc
      421 aacacatatc ctcttgcctt gacgatttac atttctttta ctcattcgcc caaagcaaat
      481 gcatctattt attttcattg tcgcttgtcc tcatgttctg ttcattcact tatgtgcgtg
      541 tgcaaa
//