LOCUS       EY195122                 318 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab17f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195122
VERSION     EY195122.1  GI:159498250
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 835 was extended to value 840 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 513
            PolyA tail found inside read...SR being truncated to 469
            Potential full insert read...vec-to-vec length: 390
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaat gctgggtctg cagttcgcct acttggagcc tgccgccgtc ggcgcccaat
       61 cggaggagca gctgctgcaa gagtgcgccg aagtgaagga cgggttcggg cgtgcggagc
      121 ggcagcagca gccgacggca gaagcccaag cgccgcagca gcagcagcag cacctcatcg
      181 cacgagtgct ccagcacatt ggcagtggcg gccgaacagc cgaaaatggc gcagacagca
      241 acagcacaca caattactgc tctttgccaa acgatgatga tccttgaaac gttgaaacag
      301 taaaaacttc tgaaaaca
//