LOCUS       EY195119                 247 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab17f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_006952.1|ND5_10020 NADH dehydrogenase subunit 5
            [Ascaris suum] sp|P24884|NU5M_ASCSU NADH-UBIQUINONE OXIDOREDUCTASE
            CHAIN 5 emb|CAA38174.1| ND5 protein [Ascaris suum], mRNA sequence.
ACCESSION   EY195119
VERSION     EY195119.1  GI:159498247
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 828 was extended to value 829 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 440
            PolyA tail found inside read...SR being truncated to 398
            Potential full insert read...vec-to-vec length: 319
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcta actcaaaaaa agatataatt gacgaaaaaa taatagtaaa taaacttaaa
       61 ataataatta aaaaataaaa attaaaatta ataaattaat tataatgtat taataaaaaa
      121 catccagcag taactaaagt tctactatgt actaaagaac taacaggtgt aggtgctctc
      181 atagctttag gtaatcatcc tataaaagga aattgagctc ttttagtaat agaaattaat
      241 tctaaca
//