LOCUS EY195113 332 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab17e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195113 VERSION EY195113.1 GI:159498241 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 332) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 846 was extended to value 850 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 522 PolyA tail found inside read...SR being truncated to 483 Potential full insert read...vec-to-vec length: 404 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..332 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca cttgccaaga atgtcttcat taatcaagaa cgaaagtcag aggatcgaag 61 gcgatcagat accgccctag ttctgaccgt aaacgatgcc aactcgcgat ccgccggcgg 121 agcatttcgc cctggtgggg agcgtcccgg aaacgaaagt cttccggttc cgggggaagt 181 atggttgcaa agctgaaact taaaggaatt gacggaaggg caccaccagt tccggaagat 241 tccttgacac cttatttttc gcaatcgagc gcatctcgca tcatttcttt tctgttgctt 301 aatttatgct ctgctaaaaa atttctcatc ga //