LOCUS       EY195112                 430 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab17e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195112
VERSION     EY195112.1  GI:159498240
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 827 was extended to value 828 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 618
            PolyA tail found inside read...SR being truncated to 581
            Potential full insert read...vec-to-vec length: 502
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt gcagaggaac acaaggaaaa agtgccaagg tcgacgagga gtgcgaactt
       61 gcttacaaaa gagtgagagg ggaaatcatt aatctgctgg acaaaacatt gaaccaaatg
      121 aagaattcgg agaccctcgt caaacttctt cacttatata aaaatgagca aaaggcattt
      181 caacatccat gccttctgtc cgacgcctta catattcaag ccaattcgaa ggcaaacaac
      241 tttgagagcc gacgtctagt tttccaacta ttctgggatt gcaaaacatg aagcaatttt
      301 attttctaat atcaattcca aattcgggtt taaaattttt aacagatgtt tgtttcctag
      361 tccagttttc cgcgctgatt atggattatg attatttgat tttatgattt aattaaatta
      421 ttatgtatca
//