LOCUS EY195092 321 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab17c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195092 VERSION EY195092.1 GI:159498220 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 321) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 841 was extended to value 841 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 513 PolyA tail found inside read...SR being truncated to 472 Potential full insert read...vec-to-vec length: 393 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..321 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaag gcttggctgg cgatgactcc agaagagcgc aaggcggtgt tgaaggcagc 61 tatcgaacag aaaaagaagg agcgtgagga caagatcaga gtgcgcaagc tgattgccaa 121 gggaaagagg ggtcagcagc gcactactgt gtccgtcttc cctaccaccg tggcagtgcc 181 catcgaaact gtcgcagcct cgtgagtgag tgaacagtct aggcgagaga aggagaaagg 241 aggaacgcat cctaaagaat gcttttttga atttcgattc acaatttgtt gttcttttta 301 aaataaaatc gcaaaagtac a //