LOCUS EY195081 218 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab17a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195081 VERSION EY195081.1 GI:159498209 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 218) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 801 was extended to value 802 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 466 PolyA tail found inside read...SR being truncated to 369 Potential full insert read...vec-to-vec length: 290 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..218 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggacc gattggcatt gccagcgacg agatcatccg ccgactgctg ggcgttgtgg 61 actacctgaa cctgatgacc tacgactatc ccagtccaga gtgaggcaaa tatacaaaaa 121 tgttgggaga caaaactggg cacatggatt tgcctttgct gatcacgaat tgtagcaaat 181 atttttatcg tatctatcga acagaaacac aggttcta //