LOCUS       EY194009                 345 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194009
VERSION     EY194009.1  GI:159498343
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 535
            PolyA tail found inside read...SR being truncated to 496
            Potential full insert read...vec-to-vec length: 417
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac acctttgtgc aagggcgccg acgtcgcgtg attgaggcgc tcaataagtg
       61 cggaatcgag ttcagggaag agccttacgt gtttgtccag caaacagaag aacgccccgg
      121 gttagagacc accgttattg tggcggaaga caccaaaatt ttcccaccgt acggaaaggt
      181 agacattgtc ggcggttcca aagctaagtg tgtgctgcgg gccctcacat ttgagaacac
      241 tttcgatgat cgcccggcct ttatgtgctg aagaagaagg gactagcggg gaggggacag
      301 tagtatccca taaccttttt gaaactacaa taaatatccg tcgga
//