LOCUS EY193993 345 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab01c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_502775.1| Putative protein family member, nematode specific [Caenorhabditis elegans] pir|T27241 hypothetical protein Y57G11C.31 - Caenorhabditis elegans emb|CAB16530.1| Hypothetical protein Y57G11C.31 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193993 VERSION EY193993.1 GI:159498327 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 345) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 538 PolyA tail found inside read...SR being truncated to 496 Potential full insert read...vec-to-vec length: 417 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..345 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcat atcagtaagc ggatggacgc acttgtaaac ccaacgcagt caaacgcgca 61 caaatagcga tggaaagggg agaggtggat gttggaggtc cggttcggct aagtatgggc 121 aggcttcaat tcaaggtcta ttggtggctg gtcgactata ttctttgcgc cccgatcatc 181 ggcccactac tcaaacccta cctcccactg gtcggctaca ttttgatgtt tacacttgca 241 tgatcaatca ataaagacgc attcattcaa tcaaaattta tgtcaccata cctaaaccac 301 caaacaaacg agaggattca ataaccaaat aaaaggatgt gatca //