LOCUS       EY193991                 464 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01c08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193991
VERSION     EY193991.1  GI:159498325
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 637 (,)
            original QR value of 844 was extended to value 845 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 784
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa cacttgggcg ttcgtggaga atttctagac gagctcgacc aattgagtgc
       61 tttcctgaac atgcgtgtgc aggatgaact ggtcgagagt gttgcatcca tctacttggc
      121 cggcattttg catcgcccgc cggaagttgg cgccgtctca atagaagagc tgaaggcgtg
      181 gctgaccgct ctcacgcaag tggtccgtga actggccgag ccgaaccgtg ttcagctgct
      241 ccgaatacga acatcgcctt atttcgtgga tgaagtagtt tcccaactgg aacagaagcg
      301 acgtctcgag gccaaatatc aacagctggg gaagatgtcc gtccaaaaac agcaacaatg
      361 cacccgagaa atcaaggaag cgcagaagtt gcttgacgca gtgatagaag cggctaaagg
      421 cctgcaggaa gagctggaga gggacttgtc gcggaggtac aacg
//